tead4 (rabbit polyclonal) antibody  (Thermo Fisher)

Journal: International Journal of Biological Sciences

Article Title: Targeting the NAT10/XIST/YAP1 Axis-Mediated Vascular Abnormalization Enhances Immune Checkpoint Blockade in Gastric Cancer

doi: 10.7150/ijbs.113325

Figure Lengend Snippet: NAT10/XIST facilitates VEGFA expression by promoting the nuclear transport of YAP1. (A) The protein expression of HIF-1α and YAP1 in nuclear and cytoplasmic component in shXIST-knockdown AGS cells (treated with 100 μM CoCl2). (B) A nucleus-cytoplasm separation assay was performed to detect the expression of YAP1 in AGS cells under normoxic conditions. (C) The location of YAP1 was detected by IF staining in shXIST GC cells. Scale bar: 100 μm. (D) The mRNA levels of CTGF and CYR61 were detected in shXIST cells by qPCR. (E) A nucleus-cytoplasm separation assay was performed to detect YAP1 expression in the nuclear and cytoplasmic components of AGS cells. (F) Representative images showing YAP1 expression (red) in Remodelin-treated GC organoids. Scale bar: 20 μm. (G-I) The mRNA and protein levels of VEGFA in shYAP1-GC cells were analyzed by qPCR (G) and western blot (I); The VEGFA protein in CM was detected by ELISA(H). (J, K) The expression of VEGFA and YAP1 proteins in shXIST- or shNAT10-AGS cells transfected with a YAP1 plasmid was detected by western blot. (L) Illustration of TEAD4 protein targeting to VEGFA promoter. (M) Dual-luciferase reporter assay was performed to confirm TEAD4 targeted to the promoter of VEGFA gene. (N) ChIP-qPCR analysis of TEAD4 binding to the VEGFA promoter region in MGC-803 and AGS cells. RNA polymerase II (RNAPII) was used as a positive control. The fold enrichment over the IgG control is represented.

Article Snippet: Immunoprecipitation was carried out using rabbit polyclonal TEAD4 antibody (Proteintech, 12418-1-AP).

Techniques: Expressing, Knockdown, Staining, Western Blot, Enzyme-linked Immunosorbent Assay, Transfection, Plasmid Preparation, Luciferase, Reporter Assay, ChIP-qPCR, Binding Assay, Positive Control, Control

Journal: PLoS ONE

Article Title: The Related Transcriptional Enhancer Factor-1 Isoform, TEAD4 216 , Can Repress Vascular Endothelial Growth Factor Expression in Mammalian Cells

doi: 10.1371/journal.pone.0031260

Figure Lengend Snippet: The previously identified TEAD4 148 isoform is also shown for comparison. Schematic indicates the TEA DNA-binding domain (TEAD), a putative nuclear localization signal (NLS), a proline rich domain (PRD) and serine-threonine-tyrosine (STY) domains.

Article Snippet: TEAD4 was visualized in tissue sections, according to a previously published indirect immunostaining method , using rabbit polyclonal anti-TEAD4 antibody (made to order by Genemed Synthesis, San Antonio, TX, see for details of custom made antibody) or, as negative control, rabbit IgG (Vector Laboratories, Burlingame, CA), at a concentration of 11 ug/mL.

Techniques: Comparison, Binding Assay

Journal: PLoS ONE

Article Title: The Related Transcriptional Enhancer Factor-1 Isoform, TEAD4 216 , Can Repress Vascular Endothelial Growth Factor Expression in Mammalian Cells

doi: 10.1371/journal.pone.0031260

Figure Lengend Snippet: The TEAD4 216 isoform can repress expression from the full length human VEGF promoter F1–R3 (p<0.01, n = 9) in contrast to the TEAD4 434 and TEAD4 148 enhancers.

Article Snippet: TEAD4 was visualized in tissue sections, according to a previously published indirect immunostaining method , using rabbit polyclonal anti-TEAD4 antibody (made to order by Genemed Synthesis, San Antonio, TX, see for details of custom made antibody) or, as negative control, rabbit IgG (Vector Laboratories, Burlingame, CA), at a concentration of 11 ug/mL.

Techniques: Expressing

Journal: PLoS ONE

Article Title: The Related Transcriptional Enhancer Factor-1 Isoform, TEAD4 216 , Can Repress Vascular Endothelial Growth Factor Expression in Mammalian Cells

doi: 10.1371/journal.pone.0031260

Figure Lengend Snippet: The TEAD4 216 isoform can repress expression from the human VEGF promoter (F2–R3) that lacks the HRE (p<0.01, n = 6). The TEAD4 148 and TEAD4 311 enchancer isoforms do not require the HRE to promote reporter gene expression (p<0.001). However the full length TEAD4 434 did not significantly enhance expression (p>0.02).

Article Snippet: TEAD4 was visualized in tissue sections, according to a previously published indirect immunostaining method , using rabbit polyclonal anti-TEAD4 antibody (made to order by Genemed Synthesis, San Antonio, TX, see for details of custom made antibody) or, as negative control, rabbit IgG (Vector Laboratories, Burlingame, CA), at a concentration of 11 ug/mL.

Techniques: Expressing, Gene Expression

Journal: PLoS ONE

Article Title: The Related Transcriptional Enhancer Factor-1 Isoform, TEAD4 216 , Can Repress Vascular Endothelial Growth Factor Expression in Mammalian Cells

doi: 10.1371/journal.pone.0031260

Figure Lengend Snippet: The TEAD4 216 isoform can competitively repress expression from the human VEGF promoter in the presence of either the TEAD4 434 or TEAD4 148 enhancer isoforms (p<0.005, n = 6).

Article Snippet: TEAD4 was visualized in tissue sections, according to a previously published indirect immunostaining method , using rabbit polyclonal anti-TEAD4 antibody (made to order by Genemed Synthesis, San Antonio, TX, see for details of custom made antibody) or, as negative control, rabbit IgG (Vector Laboratories, Burlingame, CA), at a concentration of 11 ug/mL.

Techniques: Expressing

Journal: PLoS ONE

Article Title: The Related Transcriptional Enhancer Factor-1 Isoform, TEAD4 216 , Can Repress Vascular Endothelial Growth Factor Expression in Mammalian Cells

doi: 10.1371/journal.pone.0031260

Figure Lengend Snippet: The TEAD4 216 isoform can still repress expression mediated from the human VEGF promoter (F1–R3) that includes the HRE sequence under conditions of hypoxia (p<0.001, n = 6).

Article Snippet: TEAD4 was visualized in tissue sections, according to a previously published indirect immunostaining method , using rabbit polyclonal anti-TEAD4 antibody (made to order by Genemed Synthesis, San Antonio, TX, see for details of custom made antibody) or, as negative control, rabbit IgG (Vector Laboratories, Burlingame, CA), at a concentration of 11 ug/mL.

Techniques: Expressing, Sequencing

Journal: PLoS ONE

Article Title: The Related Transcriptional Enhancer Factor-1 Isoform, TEAD4 216 , Can Repress Vascular Endothelial Growth Factor Expression in Mammalian Cells

doi: 10.1371/journal.pone.0031260

Figure Lengend Snippet: Transient plasmid transfection or stable lentiviral mediated introduction of TEAD4 216 into human cells results in reduction of native VEGF protein. Solid bars represent VEGF 165 levels, quantified by ELISA, within conditioned media collected 48 hours after transfection of pcDNA plasmid vector containing the TEAD4 216 isoform into ( A ) 293T cells, ( B ) ARPE-19 retinal pigment epithelial (RPE) cells in culture (n = 4), (p<0.05). ( C ) Lentiviral (LV) expression of the TEAD4 216 isoform in human D407 RPE cells can inhibit endogenous VEGF production. Solid bars represent VEGF 165 levels, quantified by ELISA, within conditioned media collected 48 hours after transduced RPE cells were FAC sorted and plated (n = 3),(p<0.02).

Article Snippet: TEAD4 was visualized in tissue sections, according to a previously published indirect immunostaining method , using rabbit polyclonal anti-TEAD4 antibody (made to order by Genemed Synthesis, San Antonio, TX, see for details of custom made antibody) or, as negative control, rabbit IgG (Vector Laboratories, Burlingame, CA), at a concentration of 11 ug/mL.

Techniques: Plasmid Preparation, Transfection, Enzyme-linked Immunosorbent Assay, Expressing

Journal: PLoS ONE

Article Title: The Related Transcriptional Enhancer Factor-1 Isoform, TEAD4 216 , Can Repress Vascular Endothelial Growth Factor Expression in Mammalian Cells

doi: 10.1371/journal.pone.0031260

Figure Lengend Snippet: A Cyquant cell proliferation assay determining the DNA content of cells indicate that 4 days after initial seeding of equal cell numbers the control untransduced cells proliferate at a faster rate than the LV-TEAD4 216 transduced cells (n = 8, p<0.05).

Article Snippet: TEAD4 was visualized in tissue sections, according to a previously published indirect immunostaining method , using rabbit polyclonal anti-TEAD4 antibody (made to order by Genemed Synthesis, San Antonio, TX, see for details of custom made antibody) or, as negative control, rabbit IgG (Vector Laboratories, Burlingame, CA), at a concentration of 11 ug/mL.

Techniques: CyQUANT Assay, Proliferation Assay, Control

Journal: PLoS ONE

Article Title: The Related Transcriptional Enhancer Factor-1 Isoform, TEAD4 216 , Can Repress Vascular Endothelial Growth Factor Expression in Mammalian Cells

doi: 10.1371/journal.pone.0031260

Figure Lengend Snippet: TEAD4 isoforms were expressed as fusion proteins within 293T cells with various fluorescent proteins (FP) (TEAD4 434 -Green-FP, and TEAD4 148 -Yellow-FP (pseudo-colored green) and TEAD4 216 -Green-FP) to visualize cellular localization.

Article Snippet: TEAD4 was visualized in tissue sections, according to a previously published indirect immunostaining method , using rabbit polyclonal anti-TEAD4 antibody (made to order by Genemed Synthesis, San Antonio, TX, see for details of custom made antibody) or, as negative control, rabbit IgG (Vector Laboratories, Burlingame, CA), at a concentration of 11 ug/mL.

Techniques:

Journal: PLoS ONE

Article Title: The Related Transcriptional Enhancer Factor-1 Isoform, TEAD4 216 , Can Repress Vascular Endothelial Growth Factor Expression in Mammalian Cells

doi: 10.1371/journal.pone.0031260

Figure Lengend Snippet: RT-PCR for TEAD4 from choroidal, retinal and iris tissue isolated from a non-human primate eye ( Rhesus macaque ) 24 hrs after occlusion of the central retinal artery (CRAO), indicates that the full length TEAD4 434 transcript is increased and the TEAD4 148 enhancer isoform is produced in the lasered eye. L = Lasered CRAO eye; C = control eye.

Article Snippet: TEAD4 was visualized in tissue sections, according to a previously published indirect immunostaining method , using rabbit polyclonal anti-TEAD4 antibody (made to order by Genemed Synthesis, San Antonio, TX, see for details of custom made antibody) or, as negative control, rabbit IgG (Vector Laboratories, Burlingame, CA), at a concentration of 11 ug/mL.

Techniques: Reverse Transcription Polymerase Chain Reaction, Isolation, Produced, Control

Journal: PLoS ONE

Article Title: The Related Transcriptional Enhancer Factor-1 Isoform, TEAD4 216 , Can Repress Vascular Endothelial Growth Factor Expression in Mammalian Cells

doi: 10.1371/journal.pone.0031260

Figure Lengend Snippet: Photomicrograph showing a subretinal neovascular membrane in a human ocular tissue section (Fast Red, TEAD4; hematoxylin counterstain; original magnification x 100). Insert (location indicated by rectangle; original magnification x 1000) shows positive staining for TEAD4 by vascular endothelium of a choroidal new vessel that has bridged the elastic lamina of Bruch’s membrane.

Article Snippet: TEAD4 was visualized in tissue sections, according to a previously published indirect immunostaining method , using rabbit polyclonal anti-TEAD4 antibody (made to order by Genemed Synthesis, San Antonio, TX, see for details of custom made antibody) or, as negative control, rabbit IgG (Vector Laboratories, Burlingame, CA), at a concentration of 11 ug/mL.

Techniques: Membrane, Staining